Structure-inheriting solid-state reactions under hydrothermal conditions

Solid-state NMR assessment of enzyme active center structure under nonaqueous conditions.

By using solid-state NMR spectroscopy, the integrity of the active center of alpha-chymotrypsin was investigated under a variety of nonaqueous conditions. Specifically, 13C cross-polarization/magic angle spinning NMR was used to analyze the ability of alpha-chymotrypsin to stabilize a transition state intermediate analog after freezing, drying, and addition of organic solvents (both anhydrous a...

THE PRODUCTION OF GLUCOAMYLASE BY ASPERGILLUS NIGER UNDER SOLID STATE CONDITIONS (RESEARCH NOTE)

In this study, Glucoamylase production by Aspergillus Niger was investigated under solid state conditions with low cost by-products of agricultural processes as substrate. Highest enzyme production was observed when a combination of wheat bran (WB) and corn flour (CF) was used as compared to WB+ rice bran, WB+ rice flour and WB alone. Different additions of (CF) were tested and WB+ 10% CF showe...

Speciation of Molybdates under Hydrothermal Conditions

In situ Raman spectroscopy was applied to investigate the speciation of molybdates under hydrothermal conditions in 0.2 M aqueous solution in the temperature range between 20°C and 200°C and at pH values between 7 and 1. The nature and abundance of molecular and supra-molecular species differs significantly compared to the distribution of species under ambient conditions at the same pH. Whereas...

Condensation reactions and formation of amides, esters, and nitriles under hydrothermal conditions.

Hydrothermal pyrolysis experiments were performed to assess condensation (dehydration) reactions to amide, ester, and nitrile functionalities from lipid precursors. Beside product formation, organic compound alteration and stability were also evaluated. Mixtures of nonadecanoic acid, hexadecanedioic acid, or hexadecanamide with water, ammonium bicarbonate, and oxalic acid were heated at 300 deg...

Functional diversity in templated solid state reactions